System User Guide

D5033331 A

AB SCIEX TripleTOF

5600/5600+ Instruments

39 of 116

Instrument Tuning and Calibrating

Run the Verify Performance Only option at any time; however, tune the instrument only if a loss

of sensitivity or resolution is noticed. For more information about tuning and calibration, refer to

the Advanced User Guide.

For tuning the system, use the following solutions that come with the installation kit:

For positive mode:

• For optimizing TOF MS - MSMS high resolution or MSMS High Sensitivity, use the

Tuning Solution.

• For Q1calibration, use the PPG POS solution.

In negative mode:

• For optimizing TOF MS - MSMS High Resolution or MSMS High Sensitivity, use

Taurocholic acid.

• For Q1calibration, use the PPG 3000 solution.

Required material

• Tuning solutions that are supplied in the Standards Chemical Kit shipped with the

system. If needed, a new Kit can be ordered from AB SCIEX.

• Gas-tight syringes (1.0 ml is recommended)

• PEEK (red) sample tubing

Prerequisites

• Make sure that a printer is configured.

• Make sure that the spray is stable and that the proper tuning solution is being used.

Optimize the Instrument

The following procedure shows how to verify the performance of the instrument. For more

information on using the other instrument performance options, refer to the Help.

1. In the Navigation bar, under Tune and Calibrate, double-click Manual Tuning.

2. Run a TOF MS or Product ion scan type and confirm that there is a stable TIC and

that the peaks of interest are present in the spectrum.

3. In the Navigation bar, under Tune and Calibrate, double-click Instrument

Optimization.

Note: AB SCIEX recommends that after using the Taurocholic acid, repeat

the channel alignment using the PPG 3000 solution.

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